DNA in vitro transfection: jetPEI™ (from Polyplus-transfection )
Description:
Figure: HEK-293 cells expressing GFP after transfection using jetPEI™.
Polyplus-transfection has developed a complete range of transfection reagents based on the properties of the linear cationic polyethylenimine (PEI) molecule*.
jetPEI™ transfection reagent, for reproducible transfection with low toxicity
Features/Benefits:
jetPEI™ is a unique, non-lipid, easy-to-use transfection reagent that isn't negatively affected by serum or even antibiotics. A distinctive linear polyethylenimine (PEI) derivative, jetPEI™ gently facilitates intracellular DNA delivery for high performance. It is able to do this without toxicity while successfully transfecting hundreds of different cell lines and primary cells Activity on a broad range of cells and an easy protocol make jetPEI™ ideal for routine and HTS applications.
For the complete jetPEI™ story, visit Polyplus-transfection jetPEI™.
Specifications:
| CATALOG # | DESCRIPTION | DNA DELIVERED | NUMBER OF TRANSFECTIONS |
|---|---|---|---|
| 101-01N | jetPEI™ (0.1ml) +NaCl solution | 50 µg | 50 in a 24-well plate / 16 in a 6-well/35mm plate |
| 101-05 | jetPEI™ (0.5ml) | 250 µg | 250 in a 24-well plate / 80 in a 6-well/35mm plate |
| 101-05N | jetPEI™ (0.5ml) +NaCl solution | 250 µg | 250 in a 24-well plate / 80 in a 6-well/35mm plate |
| 101-10 | jetPEI™ (1ml) | 500 µg | 500 in a 24-well plate / 160 in a 6-well/35mm plate |
| 101-10N | jetPEI™ ( ml) +NaCl solution | 500 µg | 500 in a 24-well plate / 160 in a 6-well/35mm plate |
| 101-30 | jetPEI™ (4x conc, 1ml) | 2mg | (x4) concentrated |
| 101-40 | jetPEI™ (4 x 1ml) | 2mg | 2000 in a 24-well plate / 640 in a 6-well/35mm plate |
| 101-40N | jetPEI™ +NaCl solution (4 x 1ml) | 2mg | 2000 in a 24-well plate / 640 in a 6-well/35mm plate |
ReliaBLOT® IP/Western Blot Accessory Kit (from Bethyl Laboratories, Inc. )
Description:
ReliaBLOT® IP/Western Blot reagents and procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot.
Seemless integration into existing IP/WB Procedures with
ReliaBLOT is a registered trademark of Bethyl Laboratories, Inc.
Features/Benefits:
- Dramatically reduces the usual noise of heavy chain observed when Western Blotting of IP
- Low levels traditionally obscured by the Ig heavy chain can now be observed
- No changes to your existing optimized IP protocol
- Works well when using Protein-A or Protein-G for IP
- No crosslinking required
- For use when rabbit antibodies are used in Western Blot after IP
Specifications:
| Catalog number | Size |
|---|---|
| WB120 | 20 miniblots |
| WB120T | 5 miniblots |
Worry-free Antibody Trial Program (from Assay Designs)
Description:
Worry-free Antibody Trial Program
Finding the right antibody that works for your application and species of interest can be difficult and costly. To help you identify the right antibody for your research needs, Assay Designs™ now offers a no-risk antibody trial program. Terms and condition applies
Evaluate your antibodies of choice for your application of interest, even if the species or application is not listed on the Technical Specification data sheet. If the antibody does not perform to your satisfaction, we’ll simply issue you a credit.
Please contact our staff for more detail!
Features/Benefits:
- Easily identify and evaluate your antibody of choice
- Evaluate antibody of interest for your application of choice without risk
- Select from a wide portfolio of heat shock protein, signaling, and GPCR antibodies
- Get answers from a highly-trained technical support staff