Quansys Q-Plex Cytokine Multiplex ELISA Arrays
Manufacturer: PBL InterferonSource
The Quansys Multiplex ELISA known as the Q-Plex™ technology, is a fully quantitative ELISA-based non –contact printed array test where up to 16 distinct capture antibodies from either mouse or human have been deposited to each well of a 96 or 384-well plate in a defined array. Each spot represents a distinct capture antibody population. The user just adds antigen standard and sample to individual wells of the 96-well plate and eventual results can be analyzed by imaging systems.
The Quansys Q-Plex™ Cytokine Multiplex ELISA is more efficient than the traditional kits in terms of time, cost and sample amount as up to 84 different samples can be assayed for all 16 unique cytokines in less than 2.5hrs using only one ELISA test kit with less than 30 µl of sample, compared to the 48hrs traditional kits usually take. Instead of buying 16 individual kits to run the assays, one multiplex array does it all and in turn cuts down overall cost. Current Q-Plex™ Multiplex ELISA products include six human and four mouse cytokine arrays.
Features/Benefits:
- An easy and cost-effective mean to explore cytokine profiles in samples
- No cross-reactivity among markers within the array
- Less than 30μl sample requirement
- High sensitivity - detects from 30 pg/ml to less than 1 pg/ml (analytes dependent)
- Compatible with human serum, plasma, cell culture supernatant, tissue homogenates, lysates, nasal lavage, tears and urine
- Less than three hours assay time in 96-well plate ELISA format
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Manufactured in an ISO 9000 registered facility
Specifications:
Plate Example : Viral-infected mouse tissue samples assayed using the Q-Plex™ Mouse Cytokine Array. Each individual 4 x 4 array consists of 16 distinct mouse cytokines.
Image captured by Q-View™
Traditional Vs Q-plex multiples ELISA
| Traditional ELISA | Q-Plex™ multiplex ELISA | |
| Sample Size | 50-100 μl / well | 15-30 μl / well |
| Cost Per Data Point | High Cost - $5 / data point | Lower Cost - $1 / data point |
| Speed-Data Point per Test | 96 data points / test | 1536 data points / test |
| Sensitivity | Lower Sensitivity | Higher Sensitivity |
| Product Availability | Wide Range of Products | Increasing Product Offerings |
| Protocol | Standardized | Modified |
| Time | 48 hours for 1536 data points | 3 hours for 1536 data points |
A study was conducted comparing a traditional ELISA with a Q-Plex custom array. The findings were published in February 2007 BioTechiques.
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Glow-Green Nucleic Acid Buffer
Manufacturer: eENZYME
Glow-Green is an environmentally safe, non-EtBr nucleic acid stain used for detecting double-stranded or single stranded DNA, and RNA in agarose gels. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at ~290nm and one at ~490nm.
Application
- Visualization of DNA bands as they separate during agarose gel electrophoresis
- Insolation of DNA fragments for subcloning without introducing mutations normally caused by EtBr or ultraviolet radiation
Features/Benefits:
- Sensitivity is comparable to EtBr and visualization of DNA band (>50ng) is possible under visible light
- Environmentally safe and minimizes risk to mutagens
- Accurate- reliably manufactured and quality assurance tested
- Reliable- use the same high quality accessory products used to validate Assay Designs ImmunoSet products
- Affordable- economically priced for 5 x 96 well ELISA tests
- Time Saving- prepared buffers and conveniently packaged plates take the guesswork out of the lab
- Quantitative - Intense bright dyes for enhanced detection and quantification
- Sensitive - Extremely sensitive PE conjugates
- Stable - Photostable DyLight™ 488 conjugates with quantum yield over a broad pH range (pH 4-9)
- Sensitive - only commercially available ELISA for the analysis of Hsp 70 in serum and plasma
- Time Saving - results from up to 39 samples in duplicate in just 4.5 hours
- Specific - low reactivity with other Hsp70 family members such as Hsc70 (Hsp73), Grp78, DnaK (E. coli), or Hsp71 (M. tuberculosis)
Product No |
Description |
Contents |
SB-120-0010 |
Glow-Green Nucleic Acid Buffer, 1ml |
1ml (20,000x), sufficient to prepare 20L agarose gel running buffer |
ShRNA
Manufacturer: Santa Cruz Biotechnology, Inc
ImmunoSe™ Buffer Pack & ImmunoSet™ Plate Pack
Manufacturer: Assay Designs
Assay Designs™ is happy to announce the release of new accessory packs for use with ImmunoSet products. These accessory reagents may also be used for other ELISA development projects.
Features/Benefits:
Specifications:
ImmunoSet Plate Pack
A convenient package size of clear 96 well high binding microtiter plates and plate sealers for use with any ImmunoSet product.
| Description | Quantity | Cat. # |
|---|---|---|
| ImmunoSet Plate Pack | 5 clear 96 well high-binding microtiter plates & plate sealers | 80-1930 |
| Description | Quantity | Cat. no |
|---|---|---|
| ImmunoSet Buffer Pack | 1 of each of the following | 950-003 |
| PBS Concentrate | 120 mL | 80-1927 |
| BSA Solution (10%) | 50 mL | 80-1928 |
| Tween®-20 Solutoin (10%) | 30 mL | 80-1929 |
| TMB Substrate | 50 mL | 80-1805 |
| Stop Solution 2 | 50 mL | 80-1804 |
Other accessory reagents
Assay Designs ImmunoSet products for intracellular analytes are tested with multiple lysis buffers to ensure compatability. Please refer the specific product manual for the recommended lysis buffer (e.g. RIPA Cell Lysis Buffer 2 or Extraction Reagent 1, 5x). Wash Buffer Concentrate is supplied as a large volume 20x formulation for those customers with automated plate washing equipment which requires large volumes of solution. Additional SA-HRP conjugate is available for use with biotinylated antibodies.
| Description | Quantity | Cat. #< |
|---|---|---|
| RIPA Cell Lysis Buffer | 2 100 mL | 80-1284 |
| Extraction Reagent 1, 5x | 10 mL | 80-1526 |
| Wash Buffer Concentrate, 20x | 100 mL | 80-1287 |
| SA-HRP | 12.5 µg/vial | 80-1896 |
Heat Shock Protein Monoclonal Antibody R-Phycoerythrin and DyLight™ 488 Conjugates
Manufacturer: Assay Designs
Specifications:
| Product Description | Product Type | Product Number |
|---|---|---|
| Hsp27 (G3.1), PE Conjugate | Antibodies | SPA-800PEE |
| Hsp27 (phospho-Ser78) EIA Kit | Kits | 900-165 |
| Hsp27 (phospho-Ser15) EIA Kit | Kits | 900-170 |
| Hsp40 (Hdj1) pAb, DyLight 488 Conjugate | Antibodies | SPA-400-488F |
| Hsp40 (Hdj1) pAb, PE Conjugate | Antibodies | SPA-400PEF |
| Hsp47 (Colligin) Recombinant Protein | Proteins | SPP-535F |
| Hsp60 mAb (LK-2), DyLight 488 Conjugate | Antibodies | SPA-807-488F |
| Hsp60 mAb (LK-2), PE Conjugate | Antibodies | SPA-807PEF |
| Hsp70 (Hsp72) (C92F3A-5), DyLight 488 Conjugate | Antibodies | SPA-810-488F |
| Hsp70 (Hsp72) (C92F3A-5), PE Conjugate | Antibodies | SPA-810PEE |
| Hsc70 (Hsp73) (1B5), DyLight 488 Conjugate | Antibodies | SPA-815-488E |
| Hsc70 (Hsp73) (1B5), PE Conjugate | Antibodies | SPA-815PEE |
| Hsp70 High Sensitivity ELISA Kit | Kits | EKS-715 |
| Hsp90 pAb | Antibodies | SPA-836F |
| Hsp90 beta Recombinant Protein | Antibodies | SPP-777F |
Hsp70 High Sensitivity EIA Kit
Manufacturer: Assay Designs
Assay Designs Hsp70 High Sensitivity EIA Kit is a fast and sensitive method to easily analyze serum and plasma samples from human, mouse, and rat origins. This assay allows for the specific determination of both native and recombinant Hsp70 (Hsp72). It does not detect other Hsp70 family members such as Hsc70 (Hsp73), Grp78, DnaK (E. coli), or Hsp71 (M. tuberculosis).
Features/Benefits: