Manufacturer: Polyplus-transfection
Description:
With PULSin™, you can target intracellular proteins in living cells with antibodies without fixation, eliminating the time and effort of fixing cells.
PULSin™ is an innovative reagent for the highly efficient transfer of functional proteins to the cytoplasm of living cells. PULSin™ Protein & Antibody Delivery Reagent has advantages over DNA transfection because the amount and timing of exogenous proteins in cells can be controlled. With this precise control even lethal proteins can be studied. Easily perform real time function blocking and protein localization studies using antibodies. The non-toxic process delivers proteins, with up to 98% efficiency, to a wide variety of cell lines and primary cells. Fluorescent control protein and buffer are included making PULSin™ extremely fast and easy-to-use. Simply add PULSin™ to the protein, incubate, and apply to cells.
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Specifications:
| CATALOG # | REAGENT | NUMBER OF REACTIONS |
|---|---|---|
| 501-01 | PULSin™ (0.1ml)* | 6 reactions in 6-well plates |
| 501-04 | PULSin™ (0.4ml)* | 24 reactions in 6-well plates |
| 501-16 | PULSin™ (0.4ml)* | 96 reactions in 6-well plates |
* This kit contains 20 µg of R-phycoerythrin (positive control) and 20 ml of Hepes buffer
** This kit contains 20 µg of R-phycoerythrin (positive control) and 4 x 20 ml of Hepes buffer