Quansys Q-Plex Cytokine Multiplex ELISA Arrays
Manufacturer: PBL InterferonSource
The Quansys Multiplex ELISA known as the Q-Plex™ technology, is a fully quantitative ELISA-based non –contact printed array test where up to 16 distinct capture antibodies from either mouse or human have been deposited to each well of a 96 or 384-well plate in a defined array. Each spot represents a distinct capture antibody population. The user just adds antigen standard and sample to individual wells of the 96-well plate and eventual results can be analyzed by imaging systems.
The Quansys Q-Plex™ Cytokine Multiplex ELISA is more efficient than the traditional kits in terms of time, cost and sample amount as up to 84 different samples can be assayed for all 16 unique cytokines in less than 2.5hrs using only one ELISA test kit with less than 30 µl of sample, compared to the 48hrs traditional kits usually take. Instead of buying 16 individual kits to run the assays, one multiplex array does it all and in turn cuts down overall cost. Current Q-Plex™ Multiplex ELISA products include six human and four mouse cytokine arrays.
Features/Benefits:
- An easy and cost-effective mean to explore cytokine profiles in samples
- No cross-reactivity among markers within the array
- Less than 30μl sample requirement
- High sensitivity - detects from 30 pg/ml to less than 1 pg/ml (analytes dependent)
- Compatible with human serum, plasma, cell culture supernatant, tissue homogenates, lysates, nasal lavage, tears and urine
- Less than three hours assay time in 96-well plate ELISA format
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Manufactured in an ISO 9000 registered facility
Specifications:
Plate Example : Viral-infected mouse tissue samples assayed using the Q-Plex™ Mouse Cytokine Array. Each individual 4 x 4 array consists of 16 distinct mouse cytokines.
Image captured by Q-View™
Traditional Vs Q-plex multiples ELISA
| Traditional ELISA | Q-Plex™ multiplex ELISA | |
| Sample Size | 50-100 μl / well | 15-30 μl / well |
| Cost Per Data Point | High Cost - $5 / data point | Lower Cost - $1 / data point |
| Speed-Data Point per Test | 96 data points / test | 1536 data points / test |
| Sensitivity | Lower Sensitivity | Higher Sensitivity |
| Product Availability | Wide Range of Products | Increasing Product Offerings |
| Protocol | Standardized | Modified |
| Time | 48 hours for 1536 data points | 3 hours for 1536 data points |
A study was conducted comparing a traditional ELISA with a Q-Plex custom array. The findings were published in February 2007 BioTechiques.
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